Chub-s7

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August undefined, 2024

WebMillipore 7 15 d s7 nkx6 1 insulin mafa cells. 7 15 D S7 Nkx6 1 Insulin Mafa Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed … WebMethods: ER stress was induced in post-differentiated Chub-S7 (AbdSc Ad) using tunicamycin (Tn) (0.25 μg/ml, 0.75 μg/ml) for 24 hrs, 48 hrs and 72 hrs. Assessment of mitochondrial function post Tn treatment was undertaken using the Extracellular Flux Analyser – evaluating oxygen consumption rate (OCR) and proton excretion (glycolysis ...

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WebJun 1, 2024 · Methods: Differentiated human adipocytes (Chub-S7, n=6) were treated with a broccoli extract (BE; hybrid Brassica oleracea var. italic; 10ng/ml) alone or in combination with tunicamycin (Tun; 750ng/ml), an inducer of ER stress. ER stress genes (ATF4, ATF6 and CHOP) were measured at three time points (24hr, 48hr, 72hr, n=3) using qPCR. WebOct 11, 2016 · Objective: To investigate the role of B12 and folic acid in the development of adipocyte dysfunction. Methods and Results: Microarray analysis of human adipocytes (CHUB-S7 cell line) cultured and differentiated in customised media with varying concentrations of B12 and folic acid led to the identification of two important pathways: … song id for the box

Tunicamycin-Induced Endoplasmic Reticulum Stress Mediates …

WebNov 19, 2024 · Human pre-adipocytes cell line (Chub-S7) and primary adipocytes were obtained from lean, obese and morbid obese patients, grown to confluence, differentiated for one week, maintained in nutrition media for next 7 days (day 14) and then used for further experimental analysis. In order to analyse B12 deficiency effects, customized media with ... WebApr 4, 2024 · Our results are in line with those by Gathercole et al. , who reported increased insulin-stimulated glucose uptake in a human immortalized subcutaneous adipocyte line (Chub-S7) after acute exposure to dexamethasone, as well as to hydrocortisone (up to 48 hours, in a dose- and time-dependent manner for the latter), thus proposing that the ... WebOverexpression or siRNA-mediated knockdown of endogenous C/EBPalpha significantly increased or decreased, respectively, adiponectin mRNA levels in differentiated human Chub-S7 adipocytes, while neither C/EBPbeta nor C/EBPdelta significantly affected adiponectin expression in mature adipocytes. smallest apple watch size

In vitro and ex vivo models of adipocytes American …

Chub-s7

Reconstitution of telomerase activity combined with HPV-E7 …

WebSep 1, 2024 · Methods: Human differentiated adipocytes from Chub-S7 cell line and primary abdominal subcutaneous adipocytes from lean and obese participants were treated with … WebJul 30, 2024 · We used Chub-S7, a human preadipocyte cell line that readily differentiates into adipocyte-like cells (Supplementary Fig. 1). First, given the proapoptotic action of …

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WebHuman pre-adipocyte cell line CHUB-S7 was differentiated in various B12/folate concentrations: i) control: (B12 500 nM, folate-6 μM); ii) normal B12/high folate: (B12 500 nM, folate-15, 30, 60 μM). iii) Low B12/high folate: (B12 0.15 nM, folate-6, 15, 30, 60 μM) and iv) No B12/high folate: (B12 0 nM, folate-0, 30, 60 μM). ... WebThe Chub-S7 cell line was derived from human subcutaneous primary preadipocytes which were transfected with human papillomavirus E7 oncoprotein and the human telomerase …

WebTo determine whether Chub-S7 cells were able to differenti-ate, the expression of adipocyte markers was assessed, by real-time RT-PCR, at different time points during cellular WebChevrolet Suburban K20 4WD - K20 - 1973 thru 4WD - K20 - 1980. Details. Polyurethane Body Mount Bushings. Energy's specially formulated polyurethane cab and body mounts …

WebOct 14, 2011 · The Chub-S7 cell line is a transformed human subcutaneous pre-adipocyte cell line derived from an obese female that has been validated as a good model of … WebJan 31, 2011 · Chub-s7 cell line. Proliferating chub-s7 cells were cultured in Dulbecco's MEM/Nutrient Mixture F-, DMEM-F12 (Sigma, Poole, UK) …

WebJun 1, 2005 · Chub-S7 cells are human preadipocytes immortalized by expression of human telomerase and papillomavirus E7 ( 27 ). 3T3-L1CARΔ1 cells stably express a truncated coxsackie-adenovirus receptor ( 28 ).

WebAug 22, 2003 · The immortalized Chub-S7 cells displayed a comparable proliferation rate and a similar doubling time to human primary stromal cells, 13 suggesting a limited deregulation of cell cycle checkpoints ... song id for teeth 5 seconds of summerWebNov 1, 2013 · The Chub-S7 cell line (Nestlé Research Centre, Lausanne, Switzerland) was derived from human subcutaneous adipose tissue by coexpression of human telomerase reverse transcriptase and papillomavirus E7 oncoprotein (HPV-E7) genes ( 14) and has previously been characterized in detail showing expression of typical white adipose … smallest aquarium fish in the worldWebJul 1, 2013 · Influence of SQV on mitochondrial function in Chub-S7 cells. Oxygen consumption in Chub-S7 incubated with SQV. The routine rate of respiration was determined in intact cells by high resolution respirometry, using the energy substrates present in the medium. The non-differentiated and differentiated control Chub-S7 cells … smallest arab countryWebOct 11, 2016 · Methods and Results: Microarray analysis of human adipocytes (CHUB-S7 cell line) cultured and differentiated in customised media with varying concentrations of … smallest aran islandWebNov 6, 2016 · To assess the underlying mechanism, human pre-adipocyte cell line (Chub-S7) was differentiated in various B12 concentrations (1) Control: (B12-500nM); (2) LowB12 (0.15nM) (3) Control +... song id for wolves life 3WebNov 25, 2024 · The aim of our study was to use a combined epigenomic and transcriptomic approach to identify novel genes mediating the effect of B12 on adipogenesis.Human pre … smallest apple notebookWebMay 15, 2024 · Chub-S7 adipocytes have been used for studying cellular metabolism (85), adipogenic differentiation, and miRs regulation (86). Other human preadipocyte cell lines were established through the... smallest aps c camera 2017