Diphtheria toxin dtr
WebAug 1, 2001 · Diphtheria toxin fragment A (DT-A) and attenuated DT-A have been used to study cell lineage and cell–cell interactions in the pancreas, lens, pituitary, retina and in adipose tissue 1, 2, 3, 4,... WebJan 5, 2024 · Diphtheria toxin (DT) was administered every 2 days, starting 2 days prior to tumor implantation or 2 days prior to the first antibody dose, to deplete Ccr7-proficient Xcr1-DTR + cDC1. (F) Percent change in tumor volume from the start of PTX administration in mice reconstituted with 50% Xcr1-DTR and 50% Ccr7 −/− BM. Data merged from two ...
Diphtheria toxin dtr
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WebSo far, only a CD11c cre line has been used to target ATM (Wlodarczyk et al., 2024), but was unsuccessful in depleting ATM in combination with Diphtheria Toxin strategies. In addition, a novel Spp1 tdTomato mouse line has been generated and used to monitor SPP1 from perivascular macrophages ( De Schepper et al., 2024 ), and could constitute an ... WebIn XCR1-DTRvenus mice, XCR1 + DCs can be transiently depleted by injection of diphtheria toxin, which cause cell death through human DTR. Thus, both XCR1-venus and XCR1-DTRvenus mice are useful tools to elucidate in vivo behavior and functions of XCR + DCs in lymph nodes and peripheral tissues. January 2016 Contact: Shinya Ayabe, Ph.D.
WebJul 19, 2024 · In genetically modified DTR mice used for diphtheria toxin (DT)-induced microglia depletion, the expression of the suicide gene encoding diphtheria toxin … Web13 rows · Similarly, transgenic expression of the human heparin-binding EGFR, which is the diphtheria toxin receptor (DTR), in mouse cells, renders those cells susceptible, like …
WebTo determine the role of AT-derived macrophages in cardiac remodeling after MI, a hematopoietic repopulation assay was performed, using AT-HSC or BM-HSC isolated from mice expressing the diphtheria toxin receptor (DTR) under control of the full CD11c promoter , mixed with competitor BM cells from mT/mG mice, and transplanted into … Web13 rows · Diphtheria toxin receptor binds diphtheria toxin at the EGF-like domain, and this domain is sufficient to bind diphtheria toxin, while binding of heparin-like molecules to …
WebOct 18, 2024 · Abbreviations: CC3, cleaved-caspase 3; ChAT, choline acetyltransferase; CNS, central nervous system; DTR, diphtheria toxin receptor; GCL, ganglion cell layer; GFAP, glial fibrillary acidic protein; GFP, green fluorescent protein; HB-EGF, heparin binding epidermal growth factor-like factor; MMRRC, mutant mouse regional resource center; …
WebDepletion of CD11b+ pan-MΦs in CD11b-diphtheria-toxin-receptor (DTR) mice is reported to cause implantation failure due to decreased progesterone production in the corpus luteum. However, of the M1 and M2, the type of MΦs that is … hoss\\u0027s murrysvilleWebNov 10, 2024 · This is a knock-in model in which human diphtheria toxin receptor (DTR) and EGFP are driven by endogenous Lgr5 expression, and DT administration induces rapid degeneration of Lgr5 + cells [16,22]. In the Lgr5-DTR cochlea, we found organized rows of Myosin7a + outer and inner hair cells (OHCs and IHCs) intercalated by Sox2 + SCs ( Fig … hossusanneWebMar 5, 2024 · The Rosa or iDTR (for inducible Diphtheria Toxin Receptor) mouse utilizes a transgene very similar to the Cre-driver lines. In this mouse (shown in Figure 3.7. 1 below) all cells throughout the mouse’s body carries the DNA sequence for the Diphtheria Toxin Receptor DTR). hosston louisianaWebport a novel diphtheria toxin-based system that allows to major histocompatibility complex (MHC) molecules. the inducible, short-term ablation of dendritic cells CD4-positive T helper cells recognize peptides dis-(DC) in vivo. We show that in vivo DC are required to played on MHC class II molecules while cytotoxic CD8-cross-prime CTL precursors. hoss y juke patriotsWebWe expressed the diphtheria toxin (DT) receptor in transgenic mice using a hepatocyte-specific promoter and found that injection of DT caused fulminant hepatitis. Three … hoss\u0027s murrysvilleWebMay 20, 2005 · A new system for lineage ablation is based on transgenic expression of a diphtheria toxin receptor (DTR) in mouse cells and application of diphtheria toxin … hoss y jukeWebMutation details: A targeting vector was generated so that a DTR-EGFP-pA-loxP-neo-loxP cassette (diphtheria toxin (DT) cDNA linked in-frame to EGFP polyA-floxed neo) was inserted immediately after the ATG codon in exon 1 of the Lgr5 locus (deleting the remainder of exon 1 and splice donor site in intron 1 - a 212 bp deletion) by homologous … hosszú katinka film online