Elf5-cre

Author: cifd

August undefined, 2024

WebJun 2, 2024 · Generally, longer than humans, Elf 5e has an attractive, slim physique emphasized by their long, sharp ears. However, it is a fault to consider them weak or … http://www.rpb.ioz.cas.cn/yjz/wangyanling/

Epigenetic restriction of embryonic cell lineage fate by ... - Nature

WebTherefore, in mice (Elf5-Cre knock-in), the expression of Cre recombinase driven by the Elf5 promoter can be used to study genes specifically and efficiently in the trophoblast lineage during ... WebMar 28, 2024 · Tg (Elf5-cre/ERT2,-GFP)2Rcha. Mutation details : A partial coding sequence of exon 2 of Elf5 was replaced with CreERT2-P2A-eGFP-SV40pA-Frt-Neo cassette-Frt … breakdancing images

Genome-wide search identifies - BMC Molecular Biology

WebSep 10, 2010 · To further probe the effects of loss of Elf5 during pregnancy, we compared mammary glands from K14-Cre/Elf5 f/f animals and their wildtype type littermate controls … WebApr 20, 2012 · The Elf5-null mice have been described previously . Mice of C57BL/6 strain (6–8 generations) were used for experiments with virgin mammary glands. The genotype … WebOct 5, 2008 · Epigenetic silencing of Elf5 by DNA methylation interrupts this trophoblast-specific reinforcement loop in the embryonic lineage, and thereby safeguards embryonic … costa west swindon opening times

Generation of Elf5-Cre knockin mouse strain for …

WebMay 15, 2009 · However, the Elf5 in K14-Cre/Elf5 +/f mammary tissue was expressed in a mosaic pattern (Fig. 6 E) and complete ablation of Elf5 expression was confirmed in K14-Cre/Elf5 f/f mammary epithelium (Fig. 6 I). To determine whether alterations in PrlR/Jak2/Stat5 signaling pathway are responsible for the failed lobuloalveolar … WebThe Elf5-null mice have been described previously [14]. Mice of C57BL/6 strain (6–8 generations) were used for experi-ments with virgin mammary glands. The genotype of the con-trol wild-type (WT) animals was either K14-Cre/Elf5þ/þ or Elf5f/f, whereas the genotype of the Elf5 conditional knock-outs (Elf5-null) was K14-Cre/Elf5f/f. Animal ... costa weston favellWebApr 19, 2024 · Generation of Elf5 Cre ERT 2-GFP mice. (A) Schematic diagram of the strategy followed to insert Cre-ERT2-GFP gene segment in second exon of Elf5 gene. (B) PCR image showing presence of Cre … costa wifi密码

"WebHomozygous null mice display embryonic lethality. Embryos homozygous for one null allele failed to develop extraembryonic ectoderm, did not maintain trophoblast stem cells, and had abnormal or absent mesoderm. Mice heterozygous for another allele had impaired lactation and mammary gland development. Gene Ontology. (GO) " - Elf5-cre

Elf5-cre

WebMar 13, 2024 · For this purpose, we generate a transgenic mouse with the Cre recombinase inserted into the endogenous locus of Elf5 gene that is expressed specifically in …

Did you know?

WebJun 19, 2024 · E74-like factor five (ELF5) is a basic transcription factor that plays a key role in breast tissue and gland development. However, the molecular mechanism of ELF5 in breast cancer cells has not been elucidated. In this study, we examined the effect of ELF5 on the human breast cancer cell lines MCF-7 and T47D and confirmed that ELF5 can … WebSynonym: Elf5-Cre Molecular description: CRISPR/Cas-mediated genome engineering T2A in front of the Cre coding sequence was inserted into the last coding exon of Elf5, immediately before the stop codon.

WebAug 15, 2024 · The transcription factor ELF5 (ELF5) shows robust and directionally consistent associations across different outcome definitions, including a >4-fold higher risk (odds ratio: 4.88; 95%-CI: 2.47-9.63; p-value < 5.0 × 10 -6) for severe COVID-19 per 1 s.d. higher genetically predicted plasma ELF5. We show that ELF5 is specifically expressed … WebReal-time RT–PCR analyses demonstrated that the levels of Elf5 mRNA were lower in Stat5a/5b fl/fl;Cre compared with Stat5a/5b fl/fl, and that Elf5 mRNA was induced to a greater extent by prolactin in mammary tissue of Stat5a/5b fl/fl mice as compared with Stat5a/5b fl/fl;Cre mice (Supplemental Fig. 4), indicating cell- and stage-specific ...

WebS. Singh et al. Generation of a new Elf5-Cre BAC mouse model. Results and Discussions Construct design and generation of Elf5CreERT2-GFP mice To develop Elf5CreERT2 … WebOct 5, 2008 · Epigenetic silencing of Elf5 by DNA methylation interrupts this trophoblast-specific reinforcement loop in the embryonic lineage, and thereby safeguards embryonic cells from differentiating into ...

http://www.informatics.jax.org/recombinase/specificity?id=MGI:3811269&system=renal+%26+urinary+system

http://genesdev.cshlp.org/content/23/20/2382.full costa west mallingWeb建立和稳定了包括人滋养层干细胞系、早期及足月人胎盘原代滋养层细胞、滋养层细胞-血管内皮细胞三维共培养、蜕膜dNK细胞等体外研究模型，并稳定了CYP19-Cre、ELF5-Cre介导或慢病毒/腺病毒介导的胎盘特异基因 … costa west norwoodWebJun 19, 2024 · E74-like factor five (ELF5) is a basic transcription factor that plays a key role in breast tissue and gland development. However, the molecular mechanism of ELF5 in … costa west nile streetWebMar 19, 2024 · When ELF5-6KR plus Myc-Ub were co-expressed in HEK293T cells, the mutant ELF5 protein exhibited a lower basal level of ubiquitination than its wild-type counterpart and the ELF5-6KQ mutant, an ... costa west swindonWebZero-Mix-volume-5!>> Hello everybody Ф@ jammaster is back inJ+ @ town again withJ` @ the brand-new-ever-Jф @ lasting zeromix vol5!J¶ @ diz tune was mixed to%k @ gether sun, 04/06/95=в@ mixture dun by: ----> jammaster of zeroline4U @ feeling great doing ¶@ a mix like that again T @ as u know this file0э@ belongs to the legenqC @ dary zeromix … costa west wickhamWebSynonym: Cre-ERT2, Gt (ROSA)26Sortm1 (cre/ESR1)Tyj, R26-Cre-ERT2, Rosa26CreER, Rosa26-CreERT2. Molecular description: A cassette containing Cre-ERT2 was inserted into the locus. Find mice (IMSR): Mouse Strains: 3 strains available Cell Lines: 0 lines available. Additional Tissues: break dancing instructionsWebApr 1, 2024 · Neonatal mouse kidneys deficient for Elf5 expression (Cdh16->Cre; Elf5*/f, where *= floxed or null allele) express 25% less Aqp2 and 25% less Avpr2 compared … costa west street chichester