WebScale your science for protein and biopharmaceutical characterization. Increased throughput, versatility, and ease-of-use, the Thermo Scientific™ Orbitrap™ Ascend Tribrid mass spectrometer accelerates proteomics and native protein analyses. WebOct 11, 2016 · This approach has been combined with MALDI-MS for the sequencing of DNA 12 or RNA (obtained by transcription of a DNA template in the presence of α-S-dNTPs). 13 Another protocol with DNA transcription involves a base-specific RNase digestion to generate RNA fragments for MALDI-MS 14 or a chimeric RNA/DNA can be produced by a …
Nanoelectrospray mass spectrometry/mass spectrometry for the …
WebMay 12, 2024 · Liq. chromatog. tandem mass spectrometry (LC-MS/MS) has proven to be a powerful anal. tool for the characterization of modified ribonucleic acids (RNAs). The … Using nanoflow ion-pair liquid chromatography coupled to high-resolution tandem mass spectrometry (nLC-MS/MS), we generated four datasets from RNA samples of increasing complexity. First, oligonucleotides with the sequence of mature Drosophila let-7 microRNA, 21 nt in length, were produced synthetically in … See more We developed a sequence database search engine for the identification of (modified) RNA sequences based on tandem mass spectra. The software, termed … See more In our analysis of data from the synthetic miRNA sample, we found a strong dependence of sequence coverage on the Normalized Collision Energy (NCE) value. … See more We processed the NME1 data using the three search engines Ariadne, RNAModMapper, and NASE. We ran target/decoy database searches using m5C as a variable … See more To assess the performance of our software at detecting RNA modifications, we compared the NASE search results for the NME1 lncRNA with and without NCL1 … See more shortcuts marketing login
Orbitrap LC-MS Thermo Fisher Scientific - US
WebTandem mass spectrometry, also known as MS/MS or MS 2, is a technique in instrumental analysis where two or more mass analyzers are coupled together using an additional reaction step to increase their abilities to analyse chemical samples. A common use of tandem MS is the analysis of biomolecules, such as proteins and peptides.. The … WebApr 12, 2024 · Provide mass spectrometry support to scientist in nonclinical, platform and other groups. Heres What Youll Bring to the Table. PhD +2 / MS +8 / BS/BA +10 years of relevant Industry experience with the conduct and oversight of bioanalytical methods and study support. At least 5 years of demonstrated hands-on experience with targeted LC/MS … WebMay 20, 2024 · In the 2-D mass-t R plot representing sequencing of a single ψ-containing RNA (RNA #12, which also contained a single m 5 C (5-methylcytidine)) , a new curve (red) branched up off of the original sigmoidal curve (grey) at the ψ, corresponding to the part of the sequence with all CMC-ψ adduct-containing ladder fragments, which shift up and to … shortcuts main menu